Change of hemocoagulation and peroxidation processes in patients with circulatory system diseases

There is a lot of evidence about the role of free radical oxidation in homeostasis and in the development of many diseases. Of particular interest are pathological conditions that are accompanied by both activation of free radical oxidation processes and hemocoagulation, which leads to the development of disseminated intravascular coagulation syndrome. Diseases of the circulatory system have become one of the most common causes of death worldwide, which are accompanied by activation of the hemostasis system, intravascular coagulation and free radical oxidation. Aim of our study was to investigate spontaneous luminol-dependent chemiluminescence of whole blood and hemocoagulation parameters in patients with diseases of the circulatory system. Material and methods. Free radical oxidation parameters (chemiluminescence intensity maximum, chemiluminescence intensity decay constant, and luminescence light sum) were determined using a BCLM 3606M biochemiluminometer. Free radical steady state concentration was determined using the Bouguer – Lambert – Beer formula. The rate constant for the transition of free radicals to an excited state, accompanied by the emission of light ( k 2 ), was calculated using Yu.A. Vladimirov's equation in own modification. Platelet hemostasis parameter values were determined by direct impedance measurement using a Sysmex XN-550 analyzer, coagulogram parameter ‒ using a Sysmex CA-1500 analyzer. Results. In patients with circulatory system diseases, an increase in the large platelets proportion (by 1.16 times, p = 0.05) and a decrease in the prothrombin according to Quick (by 1.22 times, p = 0.04) relative to the control group parameters are observed, an increase in the chemiluminescence intensity maximum (by 1.23 times, p = 0.05) is revealed. The steady state concentration of free radicals is 1.074·10-6 mol/dm3, the reaction rate constant is 3.068·10 6 (mol/dm 3 ) -1 ·s -1 . Conclusions. In patients with circulatory disorders, intensification of lipid peroxidation processes and activation of hemocoagulation processes are observed, as evidenced by an increase in the maximum intensity of blood spontaneous luminol-dependent chemiluminescence, the large platelets proportion and a decrease in prothrombin according to Quick. The value of the rate constant for the transition of free radicals to an excited state, accompanied by the emission of light ( k 2 ), in the blood of patients is 3.068·10 6 (mol/dm 3 ) -1 ·s -1 .