Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes

Maria J. Donde; Alicia Montulet; Alex H. Taylor

doi:10.1039/d5cb00182j

January 1, 2025RSC Chemical BiologyOpen Access

Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes

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Key Points

RNA knockdown is significantly impacted by the mismeasurement of catalysts, with implications for therapy.
Investigators found that DNAzymes and XNAzymes exhibit mismeasurement issues that can affect their efficacy in vivo.
Approach includes a comprehensive analysis of RNA-cleaving catalysts to evaluate their knockdown efficiency and potential errors.
Understanding mismeasurement aids in refining therapeutic techniques, highlighting the need for careful assessment in RNA-targeted therapies.

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Implication

Experimental analysis identifies sources of error in RNA knockdown by nucleic acid catalysts, suggesting improvements for therapeutic applications.

Abstract

RNA-cleaving olignucleotide catalysts composed of DNA and/or nucleic acid analogues (DNAzymes, modified DNAzymes and XNAzymes) are promising agents for specific knockdown of disease-associated RNAs. However, we and others have identified...

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Cite This Study

Donde et al. (2025) studied this question.

synapsesocial.com/papers/68c198be9b7b07f3a061a53b https://doi.org/10.1039/d5cb00182j

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Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes

Key Points

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Implication

Abstract

Discussion

Cite This Study

Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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