3D protein structure of chemokines in multiple sclerosis

Background. When bone marrow-derived and resident cells migrate to sites of inflammation, chemokines and their receptors play crucial roles. Immunological triggers of multiple sclerosis (MS) and the therapeutic effects of systemic treatment can be learned from peripheral blood biomarkers. This work aimed to draw the 3D protein structures of chemokines (CCL2, CCL5 and CXCL10) and observe the changes in amino acid analysis. Materials and methods. A designable study was conducted on CCL2, CCL5 and CXCL10 chemokines that related to MS in Multiple Sclerosis Center during November 2021 to May 2022. 3D protein structuring was done by sequencing of CCL2, CCL5 and CXCL10. Results. 3D protein structure of CCL2, CCL5 and CXCL10 showed a convergence between study CCL2 isolated and that of the Gen-Bank database (NCBI-2024) with identities 19/20 (95 %), CCL2 had one mutation appeared as (D to E), while there is a high convergence between study CCL5 isolated and that of the GenBank database (NCBI-2024) with identities 91/91 (100 %). A low convergence between study CXCL10 isolated and that of the GenBank database (NCBI-2024) with identities 22/28 (79 %) in CXCL10 had six mutations noticed as (C to I), (R to I), (V to Y), (E to F), (I to Y), and (I to S). Conclusions. No studies about chemokines of MS were conducted in Iraq, so the present study is found necessary as a first study to determine chemokines 3D protein structure among patients with MS in Iraq generally and in Basrah especially