Membrane reserve of ducklings' erythrocytes under the influence of ciprofloxacin in conditions of experimental staphylococcal infection

The effect of ciprofloxacin on the membrane reserve and resistance of ducklings' erythrocytes to hypotonic environment in experimental staphylococcosis was studied. The high permeability of the erythrocyte membrane to ciprofloxacin can lead to a change in the resistance of red blood cells to conditions of reduced osmolarity. The study involved three groups consisting of Bashkir ducklings aged 21 days. Group III birds were treated with ciprofloxacin at a dosage of 200 mg/l for five days. A day after the start of antibiotic treatment, intraperitoneal infection of groups II and III with Staphylococcus aureus culture at a concentration of 5McF was performed. Intracardiac puncture blood sampling was performed on the 1st, 3rd, 6th, 9th, and 13th days after infection. To assess the resistance of red blood cells to hypoosmotic pressure, blood was incubated in an isotonic solution (0.93% NaCl), in a hypotonic solution (0.33% NaCl), in a ratio of 1:1 during 30 seconds. Linear parameters of erythrocytes were determined in blood smears, surface area and volume, and indicators of membrane reserve were calculated. The relative membrane reserve of erythrocytes in birds of the control group in conditions of hypoosmolarity was the highest compared with the experimental groups. This is due to the initial increase in the size of bird cells of groups II and III under the influence of alpha-toxin St. aureus . The erythrocytes of intact ducklings demonstrate the maximum intensity of use of the relative membrane reserve in a hypotonic environment – iuMR% is in the range between 19.4% and 33%. In the second and third groups, the average value of iuMR% of the membrane is 17%. The involvement of surface plasmalemma reserves in the regulation of red blood cell size, as well as changes associated with ion and water transport, prevent hemolysis. The use of an intracellular membrane pools makes it possible to regulate the volume of nuclei under the aggressive action of alphahemolysin and ciprofloxacin.