New insights into polymorphisms in candidate genes associated with incidence to repeat breeder in Italian buffaloes (Bubalus bubalis)

This study looked at the reproductive genes that could interact with repeat breeder incidence in Italian buffaloes. Two hundred and forty female Italian buffaloes (120 repeat breeder and 120 apparently normal) were used. Blood samples were collected from each buffalo into tubes encompassing EDTA anticoagulant to isolate DNA. PCR-DNA sequencing elicited nucleotide sequence differences between normal and repeat breeder buffaloes for the reproductive (MFSD14A, PALMD, VPS13B, BMP, MTNR1A, TSHR, CSGALNACT1, CADM2, ZNF503, KRR1, MTPN, IGFBP7, LEP, and ABCC4) genes. The likelihood of dissemination of all notable nucleotide variations varied noticeably between buffalo groups with and without repeat breeders, depending on Fisher's exact test (p˂ 0.01). All of the reproductive markers under research had the exonic region mutations in repeat breeder buffaloes versus normal ones. The results may confirm the importance of these markers' nucleotide variations as candidates for repeat breeder occurrence and offer a practical buffalo management strategy.