Drug transporter (ABC), P-gp, BCRP, BSEP inhibition using drug substrate assay v1

ATP-binding cassette (ABC) transporter assays areused to study the activity and interaction of drugs with ABC transporters P-gp, BCRP, and BSEP, which are crucial for drug absorption, distribution, and excretion. These assays measure the effect of a test compound on the transport of a known probe substrate by the transporter.By quantifying the amount of substrate transported in the presence and absence of the test compound, researchers can determine the degree of inhibition. ATP-binding cassette (ABC) transporters are a family of protein pumps that move molecules across cell membranes, playing a crucial role in drug absorption, distribution, and excretion. These assays help determine if a drug is a substrate or inhibitor of a specific ABC transporter, impacting its pharmacokinetic properties and potential for drug-drug interactions.Common assay types include vesicular transport assays, cell-based uptake assays, and ATPase assays.