Liposome as Antigen Delivery System: Isolation, Preparation, Characterization

The present study was aimed on developing and characterizing Liposomal Delivery System loaded with antigen of filaria parasite extracted protein for achieving a better sustain release immunological profile. Liposomes were prepared by Reverse Phase Evaporation (REV) method with slight modification using molar ratio of Soya PC:PE:Cholesterol in different molar concentration. In this study, the stability of vesicles during storage was assessed by evaluating vesicle size, residual antigen content, and antigen integrity both before and after 30 days at temperatures of 4±1ºC and 25±1ºC. For Soya PC:PE:CH liposomes, there was only a minor increase in size when stored at 25±1ºC and negligible size change at 4±1ºC, suggesting that Soya PC:PE:CH liposomes exhibit greater stability compared to traditional Soya PC:CH liposomes. Assuming the initial antigen content as 100%, Soya PC:PE:CH liposomes experienced a loss of only 14-15% of antigen at 25±1ºC and 5-6% at 4±1ºC. Antigen integrity was assessed using SDSPAGE on the liposome formulations, specifically the optimized CL3 formulation stored at 4±1ºC for 30 days. The antigens remained intact in the formulation stored at 4±1ºC after 30 days, indicating that storage did not affect the structural integrity of the antigen. These results suggest that the liposomal antigen delivery system is promising carriers for antigen delivery and vaccine development.