Study of acute toxicity of the drug Tulamycin-AVZ

Single-dose acute toxicity studies are an important precursor to dose range determination trials.The basis of any complete toxicological study of acute toxicity is the study of potential toxic effects and the establishment of maximum allowable doses for further evaluation. According to the guidelines for acute toxicity testing, the drug compound should be administered in one or more doses for a short period of time not exceeding 24 hours, and animals should be monitored for signs of toxicity for 14 days.The purpose of our research was to study acute toxicity in rats, performed in accordance with regulatory documents. Single-dose experiments were conducted on 36 white Wistar rats. To achieve this goal, we formed 6 groups of 6 female individuals with an average body weight of 200 ± 10 g, who did not give birth and were not pregnant. In experimental animals, the test substance was administered intragastrically using a probe once in the following doses: 0.005; 0.05; 0.3; 2.0 and 5.0 g/kg of rat body weight. The control group was injected with 10% starch mucus at the rate of 1 ml per 100 g of animal. All animals were monitored, with mandatory registration of visible changes. A week after the start of the experiment, all the surviving animals were reweighed, with all the data recorded in the test report. After the experiment was completed, all the animals were subjected to humane euthanasia.As a result of the analysis of the data obtained, the LD50 of the drug Tulamycin-AVZ when administered intragastrically to rats is 2.1625 g/ kg (2162 mg/kg) of body weight. Tulamycin-AVZ according to the classification can be classified as low-toxic compounds (Class 4).