Analysis compares common methodologies for hemoglobin spectroscopy accuracy in widefield optical imaging, highlighting crosstalk issues.
Widefield optical imaging relies on accurate hemoglobin spectroscopy. Accuracy and crosstalk in this setting has been underexplored. Using the analytic solution for widefield illumination we compare performance of common simplifications and propose a novel solution.
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Forti et al. (2025) studied this question.
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