Detection of Mec-A gene in CoNS Resistant to 3rd Generation Cephalosporins Isolated from Surgical Wound Infection

Background: The risk of nosocomial infection has been estimated as two to twenty times higher in developing countries than that of developed countries. Nosocomial infections in Bangladesh exceeds 30% in some Hospitals. Objective: To Detect Mec-A gene in CoNS Resistant to 3rd Generation Cephalosporins Isolated from Surgical Wound Infection Methods: This is a Cross-sectional type of descriptive study. The study population of this study includes adult Patients of different ages and both sexes suffering from nosocomial wound infections admitted in Surgery wards, Orthopaedic wards, Gynaecology and Obstetrics wards and Burn Unit of Rajshahi Medical College Hospital. The sample size is 330 and specimens of Surgical wound swabs are collected from the patients with surgical wound infections admitted into the Departments of General Surgery, Orthopaedic Surgery, Gynaecology and Obstetrics Surgery and Burn Unit. Wound swabs were collected from the leading edge of the wound showing signs of infections. Results: A total of 330 different post-surgical wound swabs were obtained from various departments of RMCH Rajshahi for aerobic culture and sensitivity. Among these, culture yielded growth of 297 (90%) cases whereas 33 (10%) samples yielded no growth. In this study regarding antimicrobial resistance of Coagulase Negative Staphylococci, isolates show highest resistance to cefixime and ampicillin (64.58%), followed by ceftriaxone (60.42%), gentamycin (54.17%), ciprofloxacin and ceftazidime both were (52.08%). Conclusion: This study focused that the incidence rate of CoNS in surgical wound infection should not be ignored. Rather their incidence is gradually increasing. On the other hand, CoNS have emerged as a multidrug resistant pathogen bearing mecA gene. So, this pathogen should be given more emphasis especially in dealing with surgical wound infection. Indiscriminate use of antibiotics prior to susceptibility testing should be stopped immediately. Efforts should also be made to enact regulations on antibiotic usage