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September 10, 2025Applied SciencesOpen Access

Simple and Robust UPLC-MS Method for Serum Arachidonic Acid with Potential Application in Clinical Settings

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This method demonstrates high accuracy for measuring arachidonic acid in serum, indicating its potential as a biomarker for oxidative damage.

The developed UPLC-MS method achieved a linearity of R2 = 0.9952 for solvent calibration and R2 = 0.9979 for matrix calibration.
Arachidonic acid (AA) quantification showed a limit of detection of 0.046 µg/mL and a limit of quantification of 0.133 µg/mL.
The method successfully analyzed real serum samples, finding AA concentrations between 0.82 to 2.69 µg/mL.
By following ICH M10 guidelines, this method ensures reliable quantification for both research and clinical applications.

Vankova et al. (2025) studied this question.

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This method demonstrates high accuracy for measuring arachidonic acid in serum, indicating its potential as a biomarker for oxidative damage.