Media Optimization for the production of lipopeptide from Streptomyces iconiensis VR1 Strain

This study reports a suitable culture medium for lipopeptide production using different nitrogen and carbon sources. Lipopeptide was produced from Streptomyces iconiensis VR1 Strain isolated from a soil sample. Sorbitol was the most suitable carbon source, compared to glucose, starch, fructose, maltose and sucrose. The beef extract was found to be a potential source of nitrogen for lipopeptide production. Therefore, a pH of 7, temperature of 30(°C) and time (72h) showed maximum lipopeptide production yield. The lipopeptide extract obtained from streptomyces icoiniensis showed the highest antifungal activity against Mucor sp. with 70.8 ± 2.6% and Aspergillus niger 45.3 ± 1.5% of zone inhibition. Lipopeptide extract showed significant cell viability (94.68 ± 2.2%) at 75 g/mL against Human cervical HT-3 cancer cells.