Potential of N-Hexanee and N-Butanol Fractions of Kelubut Leaf (Passiflora foetida L.) on Biofilm Inhibition of Monomicrobial Pseudomonas aeruginosa and Escherichia coli

Infections caused by biofilms are significant problems because the microbial community in the biofilm matrix is highly resistant to antimicrobial agents. Pseudomonas aeruginosa and Escherichia coli are opportunistic bacteria that can cause infections in various human bodies, often involved in biofilm formation. Kelubut leaf is one of the herbs found in Indonesia and Southeast Asia. This plant has several bioactive components that have attracted attention and many have been researched, especially in the pharmaceutical field. This study aims to determine the potential of n-hexanee and n-butanol fractions in inhibiting biofilm formation from monomicrobial Pseudomonas aeruginosa and Escherichia coli. The study was conducted by extracting samples which were then fractionated and antibiofilm tests were carried out using the broth microdilution test method. The data obtained is then calculated to determine the biofilm inhibition percentage. The results showed that the N-Hexanee and N-Butanol fractions showed biofilm inhibitory activity against P. aeruginosa bacteria at 93.76%, 70.10% and on E. coli at 85.36%, 72.01%. The n-hexanee and the n-butanol fractions can inhibit biofilm formation in monomicrobial Pseudomonas aeruginosa and Escherichia coli. Isoscoparin has a better binding affinity value, so it has the potential to inhibit biofilm formation.