New Approach to Assembling Nucleic Acid Dendrons on a Solid Phase

We report the methodology to synthesize nucleic acid dendrons on a solid support via Cu(I)-catalyzed alkyne–azide cycloaddition (CuAAC). Current methods require excess coupling reagents and result in low yields. Our alternative strategy is based on standard solid-phase synthesis followed by 5′-end coupling of a multifunction linker to efficiently afford dendrons. This approach enables the synthesis of DNA/RNA dendrons whose properties are compatible with therapeutic applications.