Probiotic Fermented Milk Lactiplantibacillus pentosus Strain HBUAS53657 and Orange Juice Modulates Body Weight and Blood Glucose of Hyperglycemic Rats

ijrr.20250854Increased fasting blood glucose levels, resulting from alloxan-induced pancreatic β-cell damage can significantly alter body weight and increase oxidative stress. This study aimed to evaluate the effects of Probiotic Fermented Milk (PFM) Lactiplantibacillus pentosus strain HBUAS53657 and orange juice (OJ) on body weight (BW) and fasting blood glucose (FBG) levels in hyperglycemic rats. This experimental study used 25 male Wistar rats divided into five groups: negative control (K (-)), positive control (K (+)), PFM 1 x 108 cfu/ml (P1), PFM 1 x 109 cfu/ml (P2), and PFM 1 x 1010 cfu/ml (P3), each combined with 20% OJ for 28 days. Body weight (BW) and Fasting Blood Glucose (FBG) were measured before and after treatment. Data were analyzed using a paired t-testto compare pre- and post-treatment values of BW and FBG. The results showed PFM significantly reduced FBG in all treatment groups, with the lowest post-treatment mean observed in the P3 (105.6 ± 23.23 mg/dl, p < 0.001). BW also decreased significantly in P2 (p = 0.013) and P3 (p = 0.018). The most notable effects were found in P2, which exhibited a significant 17% reduction in BW and 74% decrease in FBG (p < 0.001). These findings suggest that probiotic fermented milk of L. pentosus HBUAS53657 and orange juice may promote metabolic health by modulating body weight and improving glycemic control in hyperglycemic rats.