Three New Pyrones With Antifungal Activity From Myrothecium verrucaria ACCC 38039

Myrothecium species have been recognized as prolific producers of biologically active secondary metabolites. Despite this, a substantial portion of their biosynthetic potential remains untapped. In this study, Myrothecium verrucaria ACCC 38039 was cultivated on a rice‐based medium at 25°C for 30 days to explore its secondary metabolite profile. Seven compounds were isolated and purified by high‐performance liquid chromatography, including three previously undescribed α‐pyrone derivatives (compounds 1 – 3 ) and four known trichothecenes (compounds 4 – 7 ). The structures of these compounds were elucidated through one‐ and two‐dimensional nuclear magnetic resonance spectroscopy and high‐resolution electrospray ionization mass spectrometry. Among these newly identified pyrones, compound 1 exhibited notable antifungal activity, with a minimum inhibitory concentration of 16 µg/mL against both Aspergillus niger and Candida albicans , underscoring its potential as a promising lead compound for antifungal drug development.