Protocol describes the expression and purification of dengue virus protease fusion construct for biochemical assays, indicating its utility in research.
This protocol details the co-expression and purification of Dengue virus serotype 2 NS2B-NS3 protease fusion construct bearing a N-terminal His-StrepII tag at small scale (<6L). The final product does not have its affinity tags removed, and is catalytically active. Final product is suitable for biophysical and biochemical assays. In the current version we added the Addgene id for the plasmid used and corrected the affiliation.
No takes yet. Share an insight, caveat, or question.
Wang et al. (2024) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: