Guidelines Bench work should be completed on the pre-PCR/DNA bench. Blue DNA-only labeled materials should be used for this protocol to avoid cross-contamination. Lab attire: gloves, lab coat, and safety glasses/goggles. Before starting Add Proof 200 ethanol to Buffer AW1 and Buffer AW2 as indicated on the reagent bottle. Preheat dry bath to 56 C. Reagent Hazards Ethanol - highly flammable, skin and eye irritant, acutely toxic. Waste Disposal All flow-through/liquid waste goes in liquid waste bucket and can be disposed of in the trash. Plastics that come into contact with tissue should be collected and disposed of in the biohazardous waste container. All other plastics (columns, tips, tubes) go in solid waste container (after pouring the liquid waste into the appropriate bucket) and can be disposed of in the trash.
Varsha Rajesh (Wed,) studied this question.