Phytochemical analysis and estimation of phytoconstituents in coded plant (222) leaves – Part VII

constituents were carried out in coded plant 222 leaves. Cold extraction method was used for the preparation of the leaf extract using ethanol as solvent. Phytochemical screening of the coded plant 222 leaf extract showed the presence of carbohydrates, steroids, phenolics and flavonoid compounds. The quantitative phytochemical analysis was carried out to estimate the total phenolic, flavonoid and condensed tannin contents. Total phenolic content, flavonoid and condensed tannin contents of coded drug 222 ethanolic extract were estimated as 107.00 mg/g Gallic Acid Equivalents, 132.54 mg/g Quercetin Equivalents and 187.90 mg/g Catechin Equivalents respectively. Chemical profiling of the extract was also carried out by Thin Layer Chromatography (TLC) and High Performance Thin Layer Chromatography (HPTLC) methods. Results of the present study suggested that the extract contains considerable amount of phenolic compounds and flavonoids and the presence of such phytocompounds is responsible for the therapeutic effects exhibited by the coded plant 222 leaves.