Experimental study of Comparison of Uro -protective efficacy of Mesna versus Hyperhydration in Cyclophosphamide-induced haemorrhagic Cystitis in Rats

Background: Haemorrhagic Cystitis (H.C) is a dose limiting side effect of cyclophosphamide (CyP). Aim: in this study we aimed to investigate the role of Mesna in the protection of CyP-induced H.C and compare its efficacy with hyper hydration. Setting and design: This animal study was conducted in the experimental Animals Breeding and Medical Research Centre of Hospital Clinic de Barcelona in the University of Barcelona. Material and Methods: Male Sprague Dawley rats (360-510 gr weight, 5 rats per group were randomly assigned to four groups): Group 1 (Control group) received no drugs, Group 11(received CyP 150mg/kg alone intraperitoneal), Group 111( received CyP150mg/kg, and mesna 150% of the CyP dose immediately and at 4 and 8 hour after administration of CyP, both drugs IV injection by intra jugular vein) ,Group 1V (received CyP150mg/kg, and hyperhydration by catheterization of the animal jugular vein with continual infusion ). Bladder of animals were assessed macroscopically and histologically 48 hours later. Gross assessment for presence of oedema and haemorrhagic and histological evaluation of damage to the bladder were scored and statistical analysis used. Results: All the animals in group 11 had evidence of HC. Significant histological damage and macroscopic changes were present in this group compared to group1 (p<0.001). The median scores of bladder damage in group111 and 1V were significantly lower compared to group 11. When the median scores for bladder damage in group 11, 111 and 1V were compared there was no significant difference among these groups. Conclusion: This study demonstrates the efficacy of mesna or hyperhydration in prevention cyclophosphamide-induced haemorrhagic cystitis