PulseTrendingJournal ClubResearchersJournalsExplore
Instagram
HomeTrendingJournal ClubExplore
Synapse
⌘+K
Synapse
September 12, 2025ChemBioChem

A Nucleotide‐Derived Fluorophore for Visualizing G‐Quadruplex Assembly and Introduction of Cation‐Regulated Activity into the Thrombin Binding Aptamer

View Full Paper
Ask AI
Bookmark
Share

Authors

RARugiya AlievaSSSvetlana SokolovaIOIlya Oleynikov

Discussion

Loading...

Member takes

Overview

Modification of nucleotides improved thrombin binding in aptamers, suggesting potential in nanodevices.

Key Points

  • A single nucleotide modification increased thrombin-binding aptamer affinity tenfold, enhancing its function.
  • The introduction of silver ions resulted in new pairing capabilities mimicking crucial base interactions for recognition.
  • Fluorescence from the modified nucleotide allowed real-time observation of conformational changes during G-quadruplex folding.
  • Kinetics of aptamer folding were analyzed using stopped-flow fluorimetry, providing insights into the modifications' effects.

Cite This Study

Alieva et al. (2025) studied this question.

synapsesocial.com/papers/68d44b3031b076d99fa549a6https://doi.org/10.1002/cbic.202500416
View Full Paper
Ask AI
Bookmark
Share