Frontiers for Development of Protein Redesign Strategy in the Era of AI

Protein design has become a central focus in contemporary biology and chemistry. This review highlights recent advances in protein redesign, a strategy that generates artificial proteins by introducing multiple mutations into template protein. We first examine sequence‑based approaches—ancestral sequence reconstruction (ASR) and consensus design (CD)—that are now widely adopted. We then discuss two next‑generation methods: (i) hybrid strategies that couple CD with structure‑guided rational design, and (ii) state‑of‑the‑art platforms that integrate generative artificial intelligence with conventional redesign workflows.