Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 14, 2025Scientific ReportsOpen Access

Sequential factor delivery enables efficient workflow for universal gene editing in clinical grade iPS cells

View Full Paper
Ask AI
Bookmark
Share

Authors

TBThomas BergerEBEkaterina BorisovaAGAnna Gamerschlag

Discussion

Loading...

Member takes

Overview

This methodology improves gene editing efficiency in induced pluripotent stem cells, suggesting broader applications in therapy.

Key Points

  • The new method achieves over 30% efficiency in knock-ins of full-length transgenes, enhancing gene editing outcomes.
  • Using sequential delivery of ribonucleoprotein complexes enables efficient identification of positive clones without compromising integrity.
  • This workflow optimizes gene editing while adhering to good manufacturing practice standards, critical for clinical use.
  • Isolated clones demonstrate maintained genomic integrity and functionality, indicating strong potential for therapeutic applications.

Cite This Study

Berger et al. (2025) studied this question.

synapsesocial.com/papers/68d44f7b31b076d99fa56d02https://doi.org/10.1038/s41598-025-17876-4
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Inducible down-regulation of MHC class I results in natural killer cell tolerance2018 · 78 citations
  2. 2cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications2015 · 216 citations
  3. 3CRISPR/Cas systems: Delivery and application in gene therapy2022 · 92 citations
  4. 4Generation of a set of isogenic, gene-edited iPSC lines homozygous for all main APOE variants and an APOE knock-out line2019 · 45 citations
  5. 5Off-target effects in CRISPR/Cas9 gene editing2023 · 611 citations