Development of the biofabrication method of silver nanoparticles based on Bacillus subtilis and study of their effect on Corynebacterium glutamicum

Aim. To develop approaches for the synthesis of silver nanoparticles using Bacillus subtilis (Ehrenberg) Cohn bacteria and to evaluate their toxicity against non-pathogenic soil microflora, particularly the bacterium Corynebacterium glutamicum (Kinoshita et al.) Abe et al. (Approved Lists). Methods. The study employed microbiological methods, in vitro cultivation techniques, agar diffusion assays, and statistical data analysis. A “green” synthesis of silver nanoparticles (AgNPs) was carried out using a matrix created from B. subtilis. Results. The possibility of extracellular AgNP synthesis using the B. subtilis B-7099 strain was demonstrated. It was found that the Ag content in the synthesized nanoparticles is approximately 66 %. It was revealed that AgNP concentrations effective against pathogenic microorganisms exhibit significantly lower toxicity against non-pathogenic soil bacteria, particularly C. glutamicum. Conclusions. Considering the ability of B. subtilis to enhance plant resistance to a range of phytopathogens, as well as the possibility of synthesizing AgNPs based on them with pronounced biological activity, the development of nanobiotechnological approaches for protecting plants from various diseases is a promising area of research.