Tectonic setting shapes microbial biosynthetic potential across global geothermal environments

Microbial communities in geothermal environments constitute an underexplored reservoir of biosynthetic gene clusters with significant biotechnological potential. Here, we investigate the secondary metabolite potential of microbial assemblages across 219 marine and continental geothermal field sites, encompassing broad environmental gradients in temperature (4.7 °C to 93.5 °C), pH (0.85 to 10.3), and tectonic setting, including convergent margins, divergent margins at mid-ocean ridges, and paleo-convergent intraplate plume systems. We identified 37,178 new biosynthetic gene clusters and demonstrated that volcanic arc systems consistently harbor the most biosynthetically diverse communities. Intraplate plume systems exhibit a terpene-enriched profile, with terpenes comprising 47 % of their biosynthetic repertoire, whereas divergent margins were dominated by nonribosomal peptide synthetases and ribosomally synthesized and post-translationally modified peptides pathways, together accounting for 64 % of their total potential. These findings position tectonics as a key driver of microbial secondary metabolism and offer a geobiological framework for guiding natural product discovery in extreme geothermal ecosystems.