Molecular MR Imaging of Neuroinflammation in Sepsis-Associated Encephalopathy: An Animal Study Using Amide Proton Transfer CEST

Motivation: The need for improved early detection of sepsis-associated encephalopathy (SAE) drives this study, addressing the limitations of current diagnostic imaging techniques. Goal(s): To evaluate if APTw imaging, supported by mpMRI, can detect early neuroinflammatory changes in a sepsis rat model, serving as a potential biomarker for SAE. Approach: A controlled rat model was used, with groups receiving varying LPS doses. APTw imaging and mpMRI were performed 24 hours post-injection to assess signal changes. Results: SEPS05 and SEPS10 groups showed significantly increased APTw and T1 signals, indicating successful identification of early inflammation markers in SAE. Impact: This study highlights APTw imaging's potential to transform early SAE diagnosis, aiding clinicians in timely intervention. It prompts further research on molecular imaging biomarkers for inflammation and opens new avenues for developing targeted therapies for sepsis-induced brain injury.