Assessment using coated blade-SPME reveals dynamics of root exudates in pea during Aphanomyces infection, indicating its role in plant-microbiome interactions.
Root exudates shape the rhizosphere and mediate plant–microbe interactions, yet traditional sampling techniques often disturb the natural environment. Here, we present the use of coated blade-solid-phase microextraction (CB-SPME) method for passive, non-invasive in situ temporal metabolomic profiling of pea ( Pisum sativum ) root exudates during infection by the soil-borne pathogen Aphanomyces euteiches. In comparison to previously established extraction techniques, CB-SPME delivered lower absolute recovery but superior reproducibility while maintaining sensitivity. This non-destructive approach preserves rhizosphere integrity, enabling continuous monitoring of dynamic metabolite fluctuations and offers new insights into how root exudate influences plant–microbiome interactions.
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Halane et al. (2025) studied this question.
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