This protocol details sterilization methods for soybean nodules, ensuring contaminant removal and preserving microbial communities.
This protocol describes thesurface sterilization and storage of root nodulesfor microbiological research, ensuring the removal ofexternal contaminantswhile maintaining viable internal microbial communities. The process includes asaline wash to remove debris,bleach sterilization, andglycerol stock preparationfor storage. The sterilized nodules are plated onLBA mediato verify sterility before downstream applications.
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Ali et al. (2025) studied this question.
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