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September 26, 2025Reproduction in Domestic Animals0 citationsOpen Access

Detection of Pathogenic LeptospiraDNA in Cervicovaginal Mucus of Mares With Reproductive Disorders in a Brazilian Herd

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DMDavide MottaOspedale MaggioreJPJuliana PedrosaUniversidade Federal FluminenseWLWalter LilenbaumUniversidade Federal Fluminense

Key Points

  • Leptospira DNA was found in 25% of mares suffering reproductive issues, indicating a potential connection to equine genital leptospirosis.
  • Among positive cases, 23.3% experienced abortions and 53.3% showed placental alterations, highlighting significant reproductive impacts.
  • Cervicovaginal mucus samples were analyzed using lip L32-PCR, demonstrating a crucial diagnostic method for detecting Leptospira.
  • These findings suggest urgent need for better awareness and diagnostics for equine genital leptospirosis in affected populations.

Abstract

ABSTRACT Leptospirosis is a zoonotic disease caused by pathogenic bacteria of the genus Leptospira . A lesser‐known form, equine genital leptospirosis (EGL), has been identified as a chronic and often silent infection involving the colonisation of the mare's genital tract. Despite its potential impact, EGL remains underdiagnosed and poorly understood, particularly in its association with reproductive inefficiency. This study showed the presence of Leptospira spp. DNA by lip L32‐PCR in the genital tract of mares with a history of reproductive disturbances. Cervicovaginal mucus samples were collected from 120 adult mares exhibiting recent reproductive problems. Results showed that 30 (25%) of the mares tested positive for Leptospira DNA. Among these 30 positive cases, 23.3% had experienced abortions, 3.3% had stillbirths, 53.3% showed placental alterations, and 36.6% were subfertile. These findings suggest a possible association between EGL and reproductive disorders in mares. The high detection rate of Leptospira DNA in genital samples reinforces the need for increased awareness and improved diagnostic efforts.

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Cite This Study

Motta et al. (2025) studied this question.

synapsesocial.com/papers/68d6cd5bb1249cec298b33e2https://doi.org/10.1111/rda.70125
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular detection of Leptospira spp. in ovarian structures of naturally infected ruminants and its potential impact on reproductive function2026
  2. 2Seroepidemiological study of leptospirosis in horses with reproductive disorders2024
  3. 3Revisiting genital leptospirosis in large animals: impacts on reproductive health, diagnostic challenges, and future directions2025
  4. 4The Role of Transplacental Infection in Leptospira spp. Epidemiology in Cattle in Caatinga Biome, Brazil2024 · 5 citations
  5. 5Association between Exposure to Leptospira spp. and Abortion in Mares in Croatia2024 · 3 citations