LAMB3 regulates ITGB4 to mediate metabolic reprogramming of lung cancer cells, thus promoting the proliferation and metastasis of cancer cells

Abstract Background Non-small cell lung cancer (NSCLC) is a common lung cancer cell type. LAMB3 promotes the progress of cancer. However, the regulatory mechanism of LAMB3 on metabolic reprogramming of NSCLC is not clear. Methods RT-qPCR and western blot were used to detect the expression level of LAMB3 in NSCLC tissues and cells. The expression level of LAMB3 in A549 and H1299 cells was changed by transfection of related vectors. Cell counting kit-8 (CCK8) was used to detect the activity of NSCLC. Transwell was evaluated the migration and invasion of cancer cells. The metabolic level changes of NSCLC cells were detected by related kits. Results Our results showed that LAMB3 and ITGB4 were highly expressed in NSCLC tissues and cells. Knocking down LAMB3 inhibited the progress of NSCLC, including cell proliferation, metastasis and metabolic level. Overexpression of ITGB4 can reverse the effects of knocking down LAMB3 on tumor progression and metabolic reprogramming. Conclusion LAMB3 regulated ITGB4-mediated metabolic reprogramming of lung cancer cells, thus promoting the proliferation and metastasis of cancer cells.