Study of the anticancer effects of microplasma treatment on PIG1 cells and A375 cells in simulated tissue fluids

In this study, human plasma-like medium (HPLM) was used to construct physiologically relevant microenvironments, and the biological effects of helium microplasma on A375 melanoma cells and PIG1 melanocytes in both cultures were systematically evaluated by comparing the conventional medium. It was shown that the plasma sensitivity of both cells in the simulated tissue fluid environment was significantly lower than that in the conventional medium environment, and the cells in this environment were more difficult to be induced to apoptosis. In the directly treated co-culture system, A375 cells accounted for more than 65 % of the total number of apoptotic cells, while PIG1 cells still maintained a high survival rate, confirming the more significant selectivity of plasma action. This research innovatively carried out the study of cell selective apoptosis by helium microplasma technology in simulating the natural cellular environment in the human body, which provides an important mechanism basis for advancing its clinical translation.