Raman spectroscopy is a widely used technology in the biomedical field, including specific applications from cancer diagnosis to an active role in the pharmaceutical industry. Despite the extensive use of Raman spectroscopy in research studies, there are still some limitations to its applicability in daily clinical diagnosis. This review initially presents the main principles of Raman spectroscopy and then its most relevant applications in the biomedical field, exploring the main advantages, challenges, and limitations. Additionally, other Raman-based techniques are identified as alternatives to the conventional technique. Overall, this review aims to present the currently available applications of Raman spectroscopy in the biomedical field and future appropriate perspectives, as possible guidance for new Raman-based biomedical devices.
Pimenta et al. (2025) studied this question.