New technique enhances single-molecule localization range using structured illumination and detection.
A new high-precision single-molecule localization scheme, ISM-FLUX, is an implementation of MINFLUX using image-scanning microscopy (ISM) with a single-photon avalanche diode (SPAD) array detector. ISM-FLUX results in a larger localization range, enhancing the robustness of the localization scheme and it also potentially enables experiments in which absorption and emission of a single fluorophore can be probed independently.
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Luciano A. Masullo (2025) studied this question.
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