Innovative strategies and prospects for synergistic enhancement of biosensor performance by CRISPR-Cas system and ferrocene tyramine

Purpose This study aims to enhance biosensor performance by integrating CRISPR-Cas systems (Cas9, Cas12a, Cas13a) with ferrocene tyramine, leveraging their specificity, programmability and signal amplification to achieve breakthroughs in sensitivity, specificity and multiplexing. Design/methodology/approach The research involves analyzing CRISPR-Cas mechanisms and ferrocene tyramine’s signal enhancement, designing experiments to test target recognition, synthesizing ferrocene tyramine and integrating both technologies into hybrid biosensors. Validation assesses sensitivity, specificity and multiplexing via controlled trials. Findings Synergy between CRISPR-Cas and ferrocene tyramine yields biosensors with remarkable sensitivity, detecting low-concentration targets with high specificity. Multiplexing is enabled by adjusting guide Ribonucleic Acids (RNAs) and ferrocene tyramine-labeled antibodies. Applications span pathogen detection, biomarker analysis and environmental monitoring. Originality/value This work innovatively combines CRISPR-Cas and ferrocene tyramine, providing a novel approach to advance biosensor technology. It offers theoretical insights and technical routes for next-gen biosensors, driving innovation in biomedicine, environmental science and food safety.