Protocol demonstrates effective cryogenic homogenisation for DNA and RNA extraction in multiple taxa, indicating higher throughput benefits.
This protocol describes the cryogenic homogenisation of tissue samples for DNA, Hi-C, or RNA extraction, intended for long-read sequencing or RNA-seq, using the MP Biomedicals FastPrep®-96. This process is highly effective for the homogenisation of tissues from certain taxonomic groups in the Tree of Life Programme, with plants, bryophytes, fungi, chordates, hard corals and arthropods are currently suitable for this method. In comparison to the Covaris cryoPREPv, the FastPrep®-96 enables lower input requirements and a higher throughput of samples. The output of this protocol are homogenised samples that can be used in any of the Sanger Tree of Life DNA and RNA extraction protocols, or in Hi-C library preparation.
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Jackson et al. (2023) studied this question.
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