Potential of Nano Phyllanthus niruri L. as Anti-DENV Candidate

Dengue fever infection, spread by the Aedes aegypti mosquitos, is cause of dengue fever. One of the home grown plants with guarantee as an antiviral operator against dengue is Phyllanthus niruri L. Be that as it may, since of the destitute steadiness and bioavailability of the dynamic fixings, its adequacy is still limited. This consider pointed to extend the dengue antiviral movement of P. niruri L. by applying a nanoencapsulation technique using κ-carrageenan matrix. P. niruri L. was nanoencapsulated using the ultrasonication technique, and its bioactive constituents were subsequently characterized. Nano P. niruri L. (Pn-NPs) is a significant advance that aims to overcome the limitations of herbal-based therapies. Pn-NPs had a particle size of 115.42 ± 23.72 nm with a loading efficiency of 99.99 ± 0.06%. The stability of Pn-NPs was better than the extract against changes in NaCl concentration, temperature and pH. P. niruri L. extract was able to inhibit dengue virus with an EC50 of 1.73 ± 0.35 μg/mL, while Pn-NPs had an EC50 degree of 0.84 ± 0.01 μg/mL. Nanoencapsulation of P. niruri L. was proven to increase the anti-DENV activity with high SI value and low cytotoxicity (CC50) compared to P. niruri L. extract.