DNA Extraction from inoculated broth for metagenomics using the Promega Maxwell RSC cultured kit v1

The Maxwell® RSC Cultured cells DNA Kit is used with the Maxwell RSC or Maxwell RSC 48 Instrument from Promega. This extraction method purifies genomic DNA (gDNA) from pure culture and/or from metagenomic enrichments efficiently using automation. The kit utilizes a silica-based paramagnetic particle that is used during the purification process. This protocol will provide information for extraction from universal enrichment broth or selective enrichment broth. Additional steps are performed when extracting DNA from gram-positive organisms. This SOP is applicable to all laboratories with the intent of obtaining high quality, purified genomic DNA or quasi-metagenomic DNA through the use of a Promega Maxwell RSC Instrument. DNA can be isolated from both gram-negative or gram-positive bacterial cells from pure culture or enrichments for use in subsequent sequencing protocols.