A Comprehensive Review on High-Performance Liquid Chromatography and Hyphenated Techniques for Analysis of Centrally Acting Skeletal Muscle Relaxants

This review provides a comprehensive overview of the analytical approaches used for the evaluation of centrally acting skeletal muscle relaxants through the application of High-Performance Liquid Chromatography (HPLC) and associated techniques. This analysis includes the evaluation of drugs in their pure form, in pharmaceutical formulations, and in human plasma or other biological fluids. A literature search was conducted focusing on a specific group of cen-trally acting skeletal muscle relaxants, such as Carisoprodol, Tizanidine, Chlorzoxazone, Diaze-pam, Clonazepam, Methocarbamol, and Baclofen. The review summarizes the application of HPLC and Ultra-Fast Liquid Chromatography (UFLC) techniques integrated with UV spectro-photometric detection and mass spectrometric refraction (LC-MS/MS) to facilitate the quantifica-tion of the above drugs. Furthermore, the validated process of analytical techniques focuses on parameters such as linearity, range, recovery, Limit of Detection (LOD), and Limit of Quantifica-tion (LOQ) to ensure reliability and precision in drug concentration evaluations. This knowledge can be utilised in further research and development, which ultimately leads to improved patient care and treatment outcomes.