Imaging peripheral nerve tissue with 3D-MUSE v1

3D-MUSE is a novel serial block-face imaging technology, designed to image tissue samples embedded in a resin medium. This protocol provides a step by step overview of imaging nerve tissues embedded in glycol methacrylate with the 3D-MUSE imaging system. The system consists of a motorized microtome for serial sectioning and infinity corrected optics with a color camera for collecting images after each sectioning cycle. Block-face images are aligned if needed to create a 3D volume stack of the sample. The 3D-MUSE imaging system is mounted on a 3-axis (XYZ) motorized stage to allow for panning and focusing of the block-face. Imaging is routinely performed at 4x or 5x magnification.