RNA extraction from organoids encapsulated in alginate norbornene using TRIzol Reagent v1

This protocol describes the isolation of high-quality RNA from organoids encapsulated in alginate norbornene hydrogels. Organoids are first released from the hydrogel matrix using alginate lyase digestion, followed by homogenization in TRIzol and RNA extraction through standard organic phase separation. The expected outcome is the recovery of intact RNA with sufficient yield and purity for downstream molecular biology applications, including qPCR and sequencing. Major optimizations include removal of the hydrogel prior to RNA extraction to minimize contamination from residual matrix components, refinement of the homogenization step to improve reproducibility, and the inclusion of glycogen blue to facilitate visualization and recovery of small RNA pellets, particularly in low-yield samples.