Trace Detection of Staphylococcal Enterotoxin B Based on Hydrogen-Terminated Diamond Solution-Gate Field-Effect Transistor

Staphylococcal enterotoxin B is the most hazardous of the various toxins produced by Staphylococcus aureus. Thus, trace detection of SEB is essential for prevention, diagnosis, and treatment. In this work, an electrochemical platform was constructed to detect SEB based on a hydrogen-terminated diamond solution-gate field-effect transistor. The connection of 1-pyrenebutyric acid-N-hydroxy succinimide ester and anti-SEB antibody on the sensing area was confirmed by atomic force microscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy. Output and transfer characteristics of the device were investigated, which obtained a wide linear response from 10–15 to 10–7 g/mL, a high sensitivity of −49.37 mV/lg [SEB concentration], and a low detection limitation of 10–15 g/mL.