Eco-Friendly synthesis of Nickel oxide Nanoparticles Using Licorice leaves extract for anticancer effect against bone cancer cells MG-63 and Antibacterial Activity

In this study, nickel oxide nanoparticles (NiO-NPs) were synthesized using nickel nitrate hexahydrate and licorice leaves extract, which served as both a reducing agent and a capping agent. The optical, structural, and microscopic properties of the NiO-NPs were characterized using a range of techniques, including UV-visible spectroscopy, Fourier transform infrared spectroscopy, energydispersive X-ray analysis, field emission scanning electron microscopy, and atomic force microscopy. The bio-reducing potential of Licorice leaves was examined using gas chromatography-mass spectroscopy for phytochemical analysis of plant extract. Furthermore, cell cytotoxicity investigation was conducted on the Mg-63 cell line derived from osteosarcoma by the MTT method .The results showed that the viability of cancer cells was reduced with increasing NiO NPs concentration up to 28.78% for MG-63 Cells at the maximum concentration (500 μg/ml) in comparison with 80.79 % for HFF cell viability as control cells, while the antimicrobial experiments have demonstrated that NiO–NP action of two types of gram-positive bacteria and for two types of gram-negative bacteria. The results showed that NiO NPs had the highest antibacterial activity at high concentrations (1024 ug/ mL), forming the largest inhibition zone against Escherichia coli, Pseudomonas aeruginosa, Staphylococci aureus and Streptococcus with inhibition zones 18mm,18mm ,15mm and 22 mm respectively.