Unlocking the potential of Vibrio natriegens for L-lysine production

L-lysine is an essential amino acid widely used for feed additive and pharmaceutical purposes. It is produced via microbial fermentation, mainly using Corynebacterium glutamicum. Recently, Vibrio natriegens has become a promising host in the bioindustry, thanks to its rapid growth, versatility in substrate utilization and high salt-tolerance. The aim of this study is to evaluate the feasibility of L-lysine production in V. natriegens. Introduction of six genes encoding core metabolic enzymes and transporter involved in lysine biosynthesis from C. glutamicum into V. natriegens genome and deletion of endogenous pckA gene enabled the production of L-lysine with a titer of 4.6 g/L (nearly 80-fold increase in comparison with the wild-type) and a yield of 152 mmol/mol glucose in the defined synthetic medium after 40 hours of fermentation in shake flask. We reported the feasibility of L-lysine biosynthesis in V. natriegens.