Antibiogram and Biofilm Formation of Enterococcus faecalis isolated from women with urinary tract infection in Baqubah city, Iraq

Enterococcus faecalis bacteria positive for Cram dye have a role causing urinary tract infections, and that 15% of urinary tract infections are caused by bacteria E. faecalis. The aims of this research are to determine the level of spread of E. faecalis and causes urinary tract infections in women, the rates of biofilm formation in local clinical E. faecalis isolates and investigate the relationship between multidrug resistances with biofilm forming ability. Between September 2023, and January 2024, 134 urine specimens were obtained from women patients in Baqubah teaching hospital, Al-Batool teaching hospital for maternity in Baqubah city. Twenty-four clinical isolates were diagnosed as E. faecalis by a percentage it reached 17.9%, Morphological, microscopical and molecular detection methods were used to diagnose the isolates. The capacity of E. faecalis isolates to form biofilm was examined using the micro-titer plate approach, the results demonstrated that all isolate produced biofilm. The method of disc diffusion was used to examine isolates sensitivity to antibiotics approach. The tested isolates exhibited 100% resistance to Erythromycin (E). while the rates of resistance ranged from Fosfomycin (FO),Rifampin(RV),Tetracycline (TE), Doxycycline(DO), Levofloxacin(LEV), Ciprofloxacin(CIP), Nitrofurantoin(F), Chloramphenicol(C), Vancomycin(VA),Ampicillin(AM),Teicoplanin (TEC) 95.83.%, 83.33%, 83.33%, 54.16%, 50%, 41.7%, 25%, 20.83%, 16.7%, 12.5%, and 8.33%, respectively