Magnetically Responsive Enzyme and Hydrogen-bonded Organic Framework Biocomposites for Biosensing

We report the one-pot synthesis of multicomponent hydrogen-bonded organic framework (HOF) biocomposites. The coimmoblization of magnetic nanoparticles (MNPs) and enzymes into the HOF crystals yielded a nano-composite biocatalytic material (MNPs-enzyme@BioHOF-1) that posses dynamic localization properties. Using a permanent magnet, it is possible to separate the MNPs-enzyme@BioHOF-1 particles from a solution. When coimmobilized with MNPs, Catalase (CAT) and glucose oxidase (GOx) showed an increased retention of their activity when coimmobilized with MNPs. MNPs-GOx@BioHOF-1 biocomposites were used to prepare a proof-of-concept glucose microfluidic biosensor, where a magnet allowed to position and keep in place the biocomposite inside a microfluidic chip. The magnetic response of these biocatalysts can pave the way to new applications for the emerging HOF biocomposites