This study investigated the potential of using waste cockleshell (CS) as a natural biocarrier for blackwater (BW) treatment. This approach seeks to address shell pollution and a sustainable, eco-friendly alternative to plastic biocarriers. The CS were analysed for surface porosity, area, volume, elements, and crystal structure. Then, the pilot-scale study confirmed biofilm growth on the shell's surface after 29 days, using a crystal violet assay and scanning electron microscopy (SEM) analysis. The free-floating plant-constructed wetland (FFP-CW) was optimally designed with a redox gradient: an aerobic-aquatic zone for nitrification and an anoxic gravel-filled zone for denitrification. The effect of integrating CS biocarriers and active aeration in FFP-CW (aquatic zone) treatment efficiency was investigated. The FFP-CW treatment efficiency for nitrate, chemical oxygen demand (COD), and total ammonia nitrogen (TAN) increased by 9.62, 6.8, and 4.83%, respectively, after integrating CS biocarriers of 0.55% filling ratio. Furthermore, the optimal dosage of 192.51 mol of oxygen in FFP-CW increased in TAN and COD removal by 31.11 and 16.54%, respectively. Analysis of the CS using an x-ray diffractometer unravelled aragonite and calcite crystalline forms at 2θ range from 10 to 70°. The stability of CS biocarriers during 427 days of BW treatment was monitored using SEM-energy-dispersive X-ray spectroscopy to assess the physical and chemical changes.
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Gogoi et al. (2024) studied this question.
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