Neonatal gut microbiota profile and the association with retinopathy of prematurity in preterm infants

Abstract Background To explore the role of gut microbiota in preterm infants at high risk of developing retinopathy of prematurity (ROP). Methods Preterm infants with gestational age (GA) < 32 weeks and/or birth weight (BW) < 1500 g born between 2020 and 2021 were prospectively enrolled. Their faecal samples were collected and analysed at different postnatal ages of life using 16S rRNA gene sequencing on the Miseq platform. The main outcome measures were the microbial diversity, taxonomy, relative abundance, bacterial predicted functional analysis, and their associations with different ROP groups. Subgroup analyses were performed by matching their GA and BW across different ROP groups. Results A total of 268 stool samples were collected from 110 preterm infants, including 13 with type 1 ROP, 44 with type 2 or mild ROP, and 53 without ROP. Type 1 ROP showed no significant difference in microbial diversity up to 8 postnatal weeks ( p = 0.057), while type 2 and no ROP groups displayed increased diversity ( p = 0.0015 and p = 0.049, respectively). Bifidobacterium genera was notably less abundant in type 1 ROP group at first postnatal week ( p = 0.022) and remained low in subsequent weeks. Predicted functional analysis revealed enriched pathways in membrane transport, carbohydrate metabolism, amino acid metabolism, and replication and repair. Conclusions Reduced gut microbial diversity may be associated with ROP development in high‐risk preterm infants. Further research is needed to comprehend how early‐life Bifidobacterium reduction affects metabolism and how targeting microbiome may help for ROP prevention and management.