Figure 4 from Therapeutic Efficacy of IL7/CCL19-Expressing CAR-T Cells in Intractable Solid Tumor Models of Glioblastoma and Pancreatic Cancer

The diversity of TCR repertoire in T cells before and after the injection of 7 × 19 CAR-T. 7 × 19 CAR-T cells generated from PBMC were sorted into CAR-positive or -negative T cells and analyzed for repertoire of TCRα and TCRβ chains (before injection). Spleen cells were harvested from the mice which rejected U87MG EGFRvIII by the injection of anti-EGFRvIII 7 × 19 CAR, 42 days after tumor inoculation. Spleen cells were sorted into CAR-positive or -negative T cells for analysis of TCRα- and TCRβ-chain repertoire (after injection). TCR repertoire was analyzed using the next-generation sequencer, and the frequencies of V and J region usage were displayed as 3D graphs. The numbers in the graphs represent the diversity index calculated as Shannon–Weaver index H (a lower number indicates a less diversity). Representative data from three samples per group are shown.