Supplementary Figure 2 from Differential Distribution of the DNA-PKcs Inhibitor Peposertib Selectively Radiosensitizes Patient-derived Melanoma Brain Metastasis Xenografts

Jianxiong Ji; Sonja Dragojevic; Cameron M. Callaghan; Emily J. Smith; Surabhi Talele; Wenjuan Zhang; Margaret A. Connors; Ann C. Mladek; Zeng Hu; Katrina K. Bakken; Paige P. Sarkaria; Brett L. Carlson; Danielle M. Burgenske; Paul A. Decker; Mohammad Abdur Rashid; Mi‐Hyeon Jang; Shiv K. Gupta; Jeanette E. Eckel‐Passow; William F. Elmquist; Jann N. Sarkaria

doi:10.1158/1535-7163.27102352

Key Points

Combined peposertib and radiation elevates the proportion of cells with high DNA damage in the M27 melanoma brain metastasis line, demonstrating substantial radiosensitization.
Elevated DNA damage was quantified specifically by measuring cells displaying >25 gH2Ax foci per cell, marking persistent double-strand breaks following combination treatment.
Bar graph quantification evaluated M27 cell responses after combined inhibitor and radiation exposure; cell line model requires further preclinical validation in vivo.

Implication

Preclinical evaluation reveals increased DNA damage in M27 melanoma cells treated with peposertib and radiation, indicating enhanced radiosensitization.

Abstract

Supplemental Figure 2 shows bar graph of quantification of cells that have >25 gH2Ax foci per cell in M27 cell line, after treatment with peposertib and radiation

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Cite This Study

Ji et al. (2024) studied this question.

synapsesocial.com/papers/68e5778bb6db6435875173f0 https://doi.org/10.1158/1535-7163.27102352

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Supplementary Figure 2 from Differential Distribution of the DNA-PKcs Inhibitor Peposertib Selectively Radiosensitizes Patient-derived Melanoma Brain Metastasis Xenografts

Key Points

Implication

Abstract

Discussion

Cite This Study

Supplementary Figure 2 from Differential Distribution of the DNA-PKcs Inhibitor Peposertib Selectively Radiosensitizes Patient-derived Melanoma Brain Metastasis Xenografts

Key Points

Implication

Abstract

Discussion

Cite This Study

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