Nuclei isolation from human brain cortex v1

This protocol can be used on flash-frozen or RNAlater-preserved brain cortex. It is based on a previously published method (1) with a few modifications including, 1) Adding Recombinant RNase Inhibitor into the EZ prep lysis buffer in the incubation steps. 2) Passing the lysate through a 40µm cell strainer after the tissue grinding. 3) Doing an additional wash. 4) Passing the nuclei suspension through a 20 µm filter before the final counting.